Article 5315

Title of the article

INFLUENCE OF GROWTH REGULATORS ON EXPRESSION OF SEX AND FORMATION OF A COMPLEX OF ECONOMICALLY USEFUL TRAITS IN MONOECIOUS HEMP PLANTS 

Authors

Serkov Valerian Aleksandrovich, Doctor of agricultural sciences, senior staff scientist, Penza Research Institute of Agriculture (1b Michurina street, Lunino village, Penza region, Russia), valerian_serkov@mail.ru
Khryanin Viktor Nikolaevich, Doctor of biological sciences, professor, sub-department of general biology and biochemistry, Penza State University (40 Krasnaya street, Penza, Russia), viktor.khryanin@gmail.com
Klimova Ludmila Vladimirovna, Research worker, Penza Research Institute of Agriculture (1b Michurina street, Lunino village, Penza region, Russia), valerian_serkov@mail.ru

Index UDK

633.522:631.527

Abstract

Background. Currently, there is a need to raise harvest of fiber and seeds and to reduce cannabinoids in inflorescences and leaves of plants. In order to improve the technology of seed-production and cultivation of hemp in conditions of the Middle Volga, the authors conducted field experiments to study the effect of growth regulators on morphological, physiological and biochemical characteristics of plants for two years.
Materials and methods. The object of study was the «Surskaya» monoecious hemp variety. Plants were treated in a phase of 3 pairs of leaves with the following solutions: gibberellic acid (GA) at concentrations of 25, 30, 50 mg/l; auxin (IAA) –10, 20 mg/l; cytokinin (BAP) – 5, 10 mg/l; chlorine-choline-chloride (CCC) –6 mg/l; Na2SeO4 – 3 mg/l, and mixtures of IAA 15 mg/l + BAP 10 mg/l; IAA 15 mg/l + CCC 6 mg/l; BAP 10 mg/l + CCC 6 mg/l. The research was carried out at the key stages of ontogeny in accordance with the «Methodical guidelines for field and greenhouse experiments with hemp» (1980). The content of cannabinoids was determined by the gas-liquid chromatographic analysis.
Results. Growth regulators had a significant effect on the morphometric characteristics of plants: overall height, technical length of the stem and length of the interstitial space. The authors noted a decrease in normal excision of male hemp plants when treated with the mixture of chlorine-choline-chloride and cytokinin. A significant increase in crop straw and fiber was noted when treated with auxin.
Conclusions. The results make it possible to recommend some growth regulators (auxins, cytokinins, chlorine-choline-chloride) for usage in hemp production in order to increase the yield of fiber and straw of monoecious hemp.

Key words

cannabis sativa, plant growth regulators, auxin, cytokinin, gibberellic acid, cannabinoids, tetrahydrocannabinol, economically valuable indication.

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References

1. Grishko N. N., Levchenko V. I., Seletskiy V. I. Genetika i selektsiya konopli: tr. VNII konopli [Genetics and selection of hemp: proceedings of All-USSR Research Institute of Hemp]. Moscow: VASKhNIL, 1937, iss. 5, pp. 73–108.
2. Demkin A. P. Len i konoplya [Flax and hemp]. 1975, no. 1, p. 17–20.
3. Demkin A. P., Bondarenko A. D. Len i konoplya [Flax and hemp]. 1979, no. 6, pp. 24–25.
4. Hoffmann W. Der Züchter [Manufacturer]. 1952, vol. 22, iss. 4, 5, pp. 147–158.
5. Sengbusch R. Zeitschrift für Pflanzenzüchtung [Journal of plant growing]. 1952, vol. 31, iss. 3, pp. 319–338.
6. Köhler D. Zeitschrift für Vererbungslehre [Journal of inheritance]. 1958, vol. 89, pp. 437–447.
7. Migal' N. D. Genetika pola konopli [Hemp gender genetics]. Glukhov: ILK, 1992, 216 p.
8. Khryanin V. N. Len i konoplya [Flax and hemp]. 1965, no. 6, pp. 33–34.
9. Khryanin V. N. S.-kh. biologiya [Agricultural biology]. 1969, no. 5, pp. 753–758.
10. Khryanin V. N., Milyaeva E. L. Doklady AN SSSR [Reports of the USSR ACADEMY of Sciences]. 1977, no. 4, pp. 982–984.
11. Khryanin V. N., Chaylakhyan M. Kh. Fiziologiya rasteniy [Plant physiology]. 1979, no. 5, pp. 1008–1015.
12. Chaylakhyan M. Kh., Khryanin V. N. Botanicheskiy zhurnal [Botanical garden]. 1980, no. 2, pp. 153–171.
13. Chaylakhyan M. Kh., Khryanin V. N. Pol rasteniy i ego gormonal'naya regulyatsiya [Plant gender and its hormonal regulation]. Moscow: Nauka, 1982, pp. 96–97.
14. Soroka V. P., Migal' N. D. V Vsesoyuz. soveshch. po embriologii rasteniy [All-USSR conference on plant embryology]. Kishinev: Shtiintsa, 1971, pp. 169–171.
15. Stepanov G. S. Biologiya, vozdelyvanie i pervichnaya obrabotka konopli i kenafa: tr.VNII lubyanykh kul'tur [Biology, cultivation and primary treatment of hemp and kenaf:proceedings of All-USSR Research Institute of Fibre Crops]. Glukhov, 1975, iss. 37, pp. 56–60.
16. Soldatov S. A., Khryanin V. N. Doklady Rossiyskoy akademii sel'skokhozyaystvennykh nauk [Reports of the Russian Academy of Agricultural Sciences]. 2006, no. 2, pp. 13–16.
17. Skoog F., Miller C. Symp. Soc. Exp. Biol. 1957, vol. 11, p. 118.
18. Kulaeva O. N. Tsitokininy, ikh struktura i funktsiya [Cytokinins, structure and function thereof]. Moscow: Nauka, 1973, 264 p.
19. Migal N. D. et al. Metodicheskie ukazaniya po provedeniyu polevykh i vegetatsionnykh opytov s konopley [Methodological guidelines to field and vegetation experiments with hemp]. Moscow: VASKhNIL, 1980, 34 p.
20. Rumyantseva L. T., Dudnik M. G. Izuchenie kollektsii konopli: metod. ukazaniya [Examination of hemp collections: methodological guidelines]. Leningrad: VNIIR, 1989, 20 p.
21. Sorokin V. I. et al. Opredelenie vida narkoticheskikh sredstv, poluchaemykh iz konopli i maka: metod. rekomendatsii. [Determination of narcotic drug types, generated from hemp and poppy: methodological recommendations]. Moscow: EKTs MVD Rossii, RFTsSE MYu Rossii, 1995, 24 p.
22. Zelenina O. N., Borodin G. I., Smirnov A. A. et al. Metodicheskie ukazaniya po otsenke soderzhaniya tetragidrokannabinola v rasteniyakh konopli metodom tonkosloynoy khromatografii [Methodological guidelines to tetrahydrocannabinol content assessment in hemp by thin-layer chromatography]. Moscow: Rossel'khozakademiya, PenzNIISKh, 2004, 12 p.
23. Raushkovskiy S. S. Metody issledovaniy pri selektsii maslichnykh rasteniy po soderzhaniyu masla [Research methods in selection of oil plants by oil content]. Moscow: Pishchepromizdat, 1959, 46 p.
24. Dospekhov B. A. Metodika polevogo opyta (s osnovami statisticheskoy obrabotki rezul'tatov issledovaniy) [Field experiment methodology (with fundamentals of research results statistical processing)]. Moscow: Agropromizdat, 1985, 351 p.

 

Дата создания: 19.01.2016 15:28
Дата обновления: 26.01.2016 13:59